| 一种香豆素荧光标记多肽的合成及应用 |
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| 引用本文： | 李燕,刘晓艳,张顺,刘亭,李勇军,兰燕宇,王爱民,王永林,何彬.一种香豆素荧光标记多肽的合成及应用[J].应用化学,2014,31(12):1413-1418. |
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| 作者姓名： | 李燕 刘晓艳 张顺 刘亭 李勇军 兰燕宇 王爱民 王永林 何彬 |
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| 作者单位： | (贵阳医学院1.基础医学院；2.民族药与中药开发应用教育部工程研究中心；
3.贵州省药物制剂重点实验室；4.药学院 贵阳 550004) |
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| 基金项目： | 国家自然科学基金资助项目(21302027);贵州省中药现代化科技产业研究开发专项项目(黔科合重G字[2013]4001);贵州省科技支撑计划社会发展攻关([2012]3079);贵阳医学院院基金博士启动基金([2014]第015号)~~ |
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| 摘 要： | Sirtuin蛋白是一类称为依赖烟酰胺腺嘌呤二核苷酸（NAD）的组蛋白去乙酰化酶，共有7个成员，均是潜在的疾病治疗靶点。 然而，目前的荧光筛选方法，只适用于SIRT1~SIRT3。 因此，根据SIRT5的新酶活，设计、合成了针对SIRT5的荧光标记多肽（ISGASE(SuK) AMC），并通过LC-MS和荧光检测证明了该荧光标记多肽能应用于SIRT5的活性筛选。
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| 关 键 词： | 组蛋白去乙酰化酶 Sirtuin SIRT5 荧光标记多肽 |
| 收稿时间： | 2014-03-24 |
| 修稿时间： | 2014-06-13 |
Synthesis and Application of a Coumarin Fluorescently Labeled Peptide |
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| LI Yan;LIU Xiaoyan;ZHANG Shun;LIU Ting;LI Yongjun;LAN Yanyu;WANG Aimin;WANG Yonglin;HE Bin.Synthesis and Application of a Coumarin Fluorescently Labeled Peptide[J].Chinese Journal of Applied Chemistry,2014,31(12):1413-1418. |
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| Authors: | LI Yan;LIU Xiaoyan;ZHANG Shun;LIU Ting;LI Yongjun;LAN Yanyu;WANG Aimin;WANG Yonglin;HE Bin |
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| Institution: | (1.College of Basic Medical Science;
2.Engineering Research Center for the Development and Application of ;
Ethnic Medicine and TCM(Ministry of Education;
3.Guizhou Provincial Key Laboratory of Pharmaceutics,Guiyang Medical College;
4.College of Pharmacy,Guiyang Medical University,Guiyang 550004,China) |
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| Abstract: | Sirtuin is a type of nicotinamide adenine dinucleotide(NAD) dependent histone deacetylase, which has seven members. It is a potential therapeutic target for many diseases. However, the present fluorescent screening method is limited to SIRT1~SIRT3. Therefore, based on the discovery of SIRT5′s novel enzymatic activity, we designed and synthesized the fluorescence labeled peptide for SIRT5, ISGASE(SuK)-AMC; and by the validations of LC-MS and fluorescence detection, the fluorescence labeled peptide can be used as a tool to perform the screening based on the enzymatic activity of SIRT5. |
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| Keywords: | histone deacetylase Sirtuin SIRT5 fluorescently labeled peptides |
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