石墨烯/铂纳米粒子杂化膜测定肾上腺素

利用循环伏安法制备了石墨烯/铂纳米粒子杂化膜修饰电极，并利用该修饰电极研究了肾上腺素（EP）的电化学行为，建立了测定肾上腺素的电化学方法。 分别利用扫描电子显微镜（SEM）和循环伏安法对电极表面的形貌和电化学性能进行了表征。 试验优化了修饰电极制备过程中影响电极性能的条件和EP的测定条件。 试验结果表明，石墨烯/铂纳米粒子修饰电极对肾上腺素有明显的电催化作用。 在pH=5.0的柠檬酸 磷酸氢二钠缓冲溶液中，EP的氧化峰电流与其浓度在4.4×10-8~2.2×10-6 mol/L的范围内呈良好的线性关系。 线性方程为ipa(10 μA)=0.0753c(mol/L)+3.7653×10-5，r=0.9989，检出限为2.2×10-9 mol/L（S/N=3）。 修饰电极表具有良好的重现性，可用于实际样品的测定。

Determination of Epinephrine by Graphene/PlatinumNanoparticle Hybrid Membrane Modified Electrode

Graphene/platinum nanoparticles hybrid membrane modified electrode was prepared by cyclic voltammetry(CV). The electrochemical behaviors of epinephrine(EP) were studied on this modified electrode, and an electrochemical method for determination of EP was developed. The morphology and the electrochemical properties of the modified electrode were characterized by scan electron microscopy(SEM) and cyclic voltammetry, respectively. The conditions that influenced the electrochemical properties of the modified electrode during the preparation processes and the conditions for determination of EP were optimized. Experimental results show that the modified electrode has excellent catalytic properties for the redox of EP. In the Na2HPO4 citric acid buffer solution(pH=5.0), the oxidative peak current of EP is linear with its concentration in the range of 4.4×10-8~2.2×10-6 mol/L. The linear regressive equation is ipa(10 μA)=0.0753c(mol/L)+3.7653×10-5 with r=0.9989, and the detection limit of 2.0×10-9 mol/L（S/N=3）. The modified electrode shows good reproducibility, high sensitivity and selectivity. It could be used for determination of the content of EP in real samples.