Femtosecond laser modification of living neuronal network |
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Authors: | Chie Hosokawa Suguru N Kudoh Ai Kiyohara Yoichiroh Hosokawa Kazunori Okano Hiroshi Masuhara Takahisa Taguchi |
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Institution: | 1.Research Institute for Cell Engineering,National Institute of Advanced Industrial Science and Technology (AIST),Osaka,Japan;2.PRESTO,Japan Science and Technology Agency,Saitama,Japan;3.Graduate School of Science,Osaka University,Osaka,Japan;4.Hamano Life Science Research Foundation,Kobe,Japan |
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Abstract: | An all-wet femtosecond laser microprocessing technique was utilized for patterning and cutting functional network of living
neuronal cells on a multi-electrode dish (MED). The neuronal cells cultured on a source substrate were transferred onto an
electrode in a MED probe in solution by utilizing a femtosecond laser-induced impulsive force and a pattern of neuronal cells
were formed on the MED probe. The cellular activity of the detached neurons was supported that neurites could be regenerated
around the electrodes. As another processing method, the neurons stretching between electrodes were selectively cut by the
direct femtosecond laser irradiation and the spontaneous electrical activity of the neuronal network was evaluated. While
the spontaneous action potentials of neurons were synchronized before the cutting, the synchronization disappeared after the
cutting, indicating that the neuronal network is locally disconnected by the laser cutting. The present method is applicable
to artificial reconstruction of living neuronal network. |
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Keywords: | PACS" target="_blank">PACS 42 62 Be 87 19 Lj 52 38 Mf |
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