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REVERSIBLE BLEACHING OF Chlamydomonas reinhardtii RHODOPSIN in vivo
Authors:P Hegemann    U Hegemann  K W Foster
Institution:Max-Planck-Institut für Biochemie Am Klopferspitz 18a, 8033 Martinsried, W. Germany;Physics Department, Syracuse University, Syracuse, NY,13244–1130, USA
Abstract:Abstract— The effect of hydroxylamine on the phototactic activity of Chlamydomonas reinhardtii was investigated. The following results were obtained: (1) wild type cells, irradiated for 10 min with green light immediately after addition of 1 mM hydroxylamine, showed a 20 min transient loss of phototactic activity, (2) irradiation of cells, preincubated in the dark with 4 mM. hydroxylamine for 30 min, diminished the phototactic sensitivity permanently by more than 100-fold without loss of cell motility. (3) The phototactic sensitivity completely recovered within 3(1 min of the removal of hydroxylamin from carotenoid-containing cells or from carotenoid-negative cells upon addition of 11- cis or all- trans retinal. Our explanation is bleaching of rhodopsin by more than 99% and reconstitution by de novo synthesized or by added retinal.
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