| 紫红素-18-酰亚胺的合成及其对牛血清白蛋白结合作用的荧光法研究 |
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| 引用本文： | 王进军,刘永明,李利.紫红素-18-酰亚胺的合成及其对牛血清白蛋白结合作用的荧光法研究[J].光谱学与光谱分析,2005,25(4):594-597. |
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| 作者姓名： | 王进军 刘永明 李利 |
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| 作者单位： | 烟台大学化学院，山东 烟台 264005 |
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| 基金项目： | 国家科技部中韩政府间合作项目 (2 0 0 2 )资助 |
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| 摘 要： | 合成表征了化合物紫红素-18-酰亚胺。用荧光光谱法、分光光度法研究了水溶液中紫红素-18-酰亚胺与牛血清白蛋白(BSA)的相互结合反应。实验表明紫红素-18-酰亚胺与牛血清白蛋白的相互结合作用为单一的静态猝灭过程。在水溶液中紫红素-18-酰亚胺与蛋白质以摩尔比1∶1牢固结合，其结合常数k=5.386×105 L·mol-1。根据Frster非辐射能量转移机理，求算了给体(BSA)与受体(RPA)间距离r=3.54 nm能量转移效率E=0.26。
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| 关 键 词： | 紫红素-18-酰亚胺 牛血清白蛋白 荧光猝灭 结合反应 |
| 文章编号： | 1000-0593(2005)04-0594-04 |
| 收稿时间： | 2003-08-26 |
| 修稿时间： | 2003年8月26日 |
Synthesis of Purpure-18-Imide and Study on Its Interaction with Bovine Serum Albumins by Fluorescence |
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| WANG Jin-jun,LIU Yong-ming,LI Li.Synthesis of Purpure-18-Imide and Study on Its Interaction with Bovine Serum Albumins by Fluorescence[J].Spectroscopy and Spectral Analysis,2005,25(4):594-597. |
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| Authors: | WANG Jin-jun LIU Yong-ming LI Li |
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| Institution: | College of Chemistry, Yantai University, Yantai 264005, China |
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| Abstract: | Purpure-18-imide was synthesized based on the porphyrin's special affinity for cancer cells and the antitumor activity of pyrrolidine compound. The binding reaction between purpure-18-imide and bovine serum albumins (BSA) in aqueous was studied by fluorescence and UV-Vis absorption spectra. The research results indicated that the combination reaction of them was a single static quenching process. In aqueous, purpure-18-imide strongly bound BSA with molar ratio of 1:1. The binding constant K0 was 5.386 x 10(5) L x mol(-1). The shortest binding distance (r = 3.54 nm) and energy transfer efficiencies (E = 0.26) between donor (BSA) and acceptor (purpure-18-imide) were obtained by Forster's nonradiative energy transfer mechanism. |
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| Keywords: | Purpure-18-imide BSA Fluorescence quenching Binding reaction |
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