Isolation of plasma lipoproteins by a combination of differential and density gradient ultracentrifugation

Summary A method for preparative isolation of serum lipoproteins by a combination of differential and density gradient ultracentrifugation is presented. Total plasma lipoproteins are first isolated in a concentrated form by ultracentrifugation in a fixed angle rotor at a plasma background density of 1.21 kg/l. Subsequently, the various lipoprotein classes are separated by density gradient ultracentrifugation in a swinging bucket rotor. The procedure requires only two ultracentrifugation steps and combines advantages of both ultracentrifugation techniques.

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Isolierung von Plasmalipoproteinen durch eine Kombination von Differential- und Dichtegradient-Ultrazentrifugation

Abbreviations VLDL very low density lipoproteins - LDL low density lipoproteins - HDL high density lipoproteins - VHDL very high density lipoproteins