Peak Multiphoton Excitation of mCherry Using an Optical Parametric Oscillator (OPO) |
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| Authors: | Tegy J Vadakkan James C Culver Liang Gao Tiemo Anhut Mary E Dickinson |
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| Institution: | (1) Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA;(2) Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA;(3) Department of Bioengineering, Rice University, Houston, TX 77005, USA;(4) Carl Zeiss MicroImaging GmbH, Carl-Zeiss-Promenade 10, 07745 Jena, Germany;(5) Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030, USA |
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| Abstract: | mCherry is a red fluorescent protein which is bright, photostable, and has a low molecular weight. It is an attractive choice
for multiphoton fluorescence imaging; however, the multiphoton excitation spectrum of mCherry is not known. In this paper
we report the two photon excitation spectrum of mCherry measured up to 1190 nm in the near infrared (NIR) region. Skin tissues
of transgenic mice that express mCherry were used in the experiments. mCherry in the tissues was excited with a Titanium:Sapphire
laser and an optical parametric oscillator pumped by the Titanium:Sapphire laser. We found that the peak excitation of mCherry
occurs at 1160 nm. |
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