Establishment of the Model CD40 Cell Membrane Chromatography and Its Chromatographic Characteristics

A new model of atherosclerosis cell membrane chromatography has been established by using a CD40 cell membrane stationary phase (CD40 CMSP) prepared by immobilizing the CD40 cell membrane onto the surface of a silica carrier. The surface and chromatographic characteristics of CD40 CMSP were studied. The retention characteristics of anti-CD40 antibody and statins (lovastatin, simvastatin and pravastatin) were also investigated using this model. Affinities of the anti-CD40 antibody and statins toward CD40 cell membrane and receptors were based on the determination of log k′ values (the logarithm of capacity factor of a solute). There was a significant correlation between the affinity in the CD40–CMC and the effect in vitro for the pharmacological effect.