LC Quantification of RT-B: The Active Metabolite of a New Resveratrol Derivative RT-A in Rat Plasma |
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Authors: | Jianyang Lin Yang Liu Xiaohui Chen Kaishun Bi |
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Institution: | 1. School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, 110016, Shenyang, Liaoning, People’s Republic of China 2. Department of Pharmacy, The First Affiliated Hospital of China Medical University, 110001, Shenyang, People’s Republic of China
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Abstract: | RT-A, a new prodrug based on resveratrol, is currently under investigation. Preclinical studies in rats indicate that RT-A is readily absorbed and rapidly split into an active metabolite RT-B by lysase of the ester bond. An LC method was developed for the determination of RT-B in rat plasma. The assay was performed on a 5 μm Elite C18 column (200 mm × 4.6 mm) with a mobile phase consisting of acetonitrile–0.1% phosphoric acid (28:72, v/v, pH 1.8) at a flow rate of 1.0 mL min?1. Detection was at 318 nm, and baicalin was used as an internal standard. Calibration was linear over the range of 0.04–10 μg mL?1 with a correlation coefficient of 0.9994. The mean extraction recoveries of RT-B determined over the concentrations of 0.1, 1.0, and 5.0 μg mL?1 were (86.5 ± 6.8) %, (82.6 ± 2.0) %, and (92.7 ± 7.9) %. The RSD of intra- and inter-day precisions were all less than 10%. This method was successfully applied to evaluate the pharmacokinetics of RT-B after intravenous administration of RT-A. |
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