LC Analysis of Lignans from Schisandra sphenanthera Rehd. et Wils. |
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Authors: | Wei Gu Nanyu Wei Zhezhi Wang |
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Institution: | 1. Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi’an, Shaanxi, 710062, People’s Republic of China 2. College of Life Sciences, Shaanxi Normal University, Xi’an, Shaanxi, 710062, People’s Republic of China
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Abstract: | Schisandra sphenanthera Rehd. et Wils. is widely used in traditional Chinese medicine. A rapid and convenient method to separate and quantify four lignans (schisandrin, schisantherin A, deoxyschizandrin, and γ-schizandrin) was established by reversed-phase liquid chromatographic. On a Shimadzu C18 column (Phenomenex, 150 × 4.6 mm; 5 μm particle size), an isocratic flow elution program and a simplified sample pretreatment approach were used in the experiment. Samples from different parts of S. sphenanthera were extracted by chloroform and then separated with methanol and deionized water (70:30 v/v) at a flow rate of 0.8 mL min?1. The detection wavelength was set at 280 nm. The content of lignans in fruits is the highest, and the quantities of schisantherin A, deoxyschizandrin, and γ-schizandrin from fruits are 0.56, 0.54 and 0.30%, respectively. Schisandrin is not detected in all the plant extracts. This research forms a basic framework for the better use of S. sphenanthera in medicine. |
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