Simultaneous Determination of Pyridoxine, Meclizine and Buclizine in Dosage Formulations and Human Serum by RP-LC |
| |
Authors: | Muhammad S Arayne Najma Sultana Farhan A Siddiqui |
| |
Institution: | 1. Department of Chemistry, University of Karachi, Karachi, 75270, Pakistan 2. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Karachi, Karachi, Pakistan
|
| |
Abstract: | Rapid liquid chromatographic procedures for analytical quality control of pharmaceutical preparations and human serum containing antihistamine drugs, meclizine and buclizine alone or in combination with pyridoxine are proposed, using acetonitrile:water (80:20) as a mobile phase (pH adjusted to 2.6), methylparaben as internal standard and UV detection was made at 230 nm. The results obtained showed a good agreement with the declared content. The method shows good linearity in the range of 30–10,000 ng mL?1 for pyridoxine and 25–10,000 ng mL?1 for meclizine and buclizine serum concentrations with a correlation coefficient 0.9999 (inter- and intra-day CV < 3.91%). The recovery was >97.8%. The proposed method may be used for the quantitative analysis of meclizine and buclizine alone or in combination with pyridoxine from raw materials, in bulk drugs, dosage formulations and in serum. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|