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LC with Fluorimetric Detection for Sensitive Analysis of Reduced Flubendazole in Biological Samples |
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| Authors: | Vladimír Kubíček Marie Soukupová Milan Nobilis Veronika Křížová Barbora Szotáková Lenka Skálová |
| Affiliation: | 1. Department of Biophysics and Physical Chemistry, Faculty of Pharmacy, Charles University Prague, Heyrovského 1203, 500 05, Hradec Kralove, Czech Republic 2. Institute of Experimental Biopharmaceutics, Joint Research Centre of PRO.MED.CS a. s. Prague and the Academy of Sciences of the Czech Republic, Heyrovského 1207, 500 03, Hradec Kralove, Czech Republic 3. Department of Biochemical Sciences, Faculty of Pharmacy, Charles University Prague, Heyrovského 1203, 500 05, Hradec Kralove, Czech Republic |
| Abstract: | A validated LC method is proposed for analysis of flubendazole and its metabolites in biological samples of Haemonchus contortus. Two detectors were used—photodiode-array and spectrofluorimetric. The native fluorescence of reduced flubendazole, the key substance investigated during biological experiments, was used for its fluorimetric detection with a very low limit of quantification (0.63 nmol L?1). |
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