Bufferized solvent extraction and HPLC fluorometric detection method for sarafloxacin in pig and chicken muscles |
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| Authors: | Jeong‐Heui Choi Tae Woong Na Md Iqbal Rouf Mamun A M Abd El‐Aty Eun‐Ho Shin Jong‐Hyouk Park Jae‐Han Shim |
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| Institution: | 1. Natural Products Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University, 77 Yongbong‐ro, Buk‐gu, Gwangju 500‐757, Republic of Korea;2. Department of Chemistry, University of Dhaka, Dhaka‐1000, Bangladesh;3. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211‐Giza, Egypt |
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| Abstract: | In this study, a method for the detection of sarafloxacin in pig and chicken muscles was developed using HPLC‐FLD as a regulatory residue technique. Good extraction efficiency was achieved using a mixture of 1% orthophosphoric acid–0.2 m MgCl2 in water and acetonitrile as an extraction solvent, and n‐hexane partitioning and centrifugation for cleanup was used in the absence of dehydration. Specificity, linearity, detection and quantification limits, recovery, accuracy and precision were all validated, and all results were sufficient for the SARA regulatory residue method in pig and chicken muscles. The method developed and described herein was not only simple but also reliable, and was applied to market samples to determine their residue contents. Copyright © 2010 John Wiley & Sons, Ltd. |
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| Keywords: | sarafloxacin HPLC‐FLD pig chicken muscle |
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