Zur lokalisierung funktioneller gruppen in steroiden mit hilfe der massenspektrometrie X : 5-Hydroxy-cholestane und 3,5-dihydroxy-cholestane sowie deren acetylderivate

Mass spectra of steroids with hydroxygroups in position 3 and 5 are characterized by ions produced by elimination of C-1 to C-4. These degradation reactions are especially important for steroids with a 5β-configurated hydroxygroup.In addition 3α,5β-dihydroxysteroids loose the carbon atoms of ring A together with C-6 in a structure specific cleavage process, enabling an unambigiuous determination of the configuration. Apart from these degradation reactions caused by the presence of functional groups derivatives of cholestanes show the well known cleavage processes of ring D. 3α,5-Dihydroxycholestanes may be differentiated from their 3β-isomeres by the preferential production of an ion of mass 247.