Proteomics‐based Characterization of Protein Complexes from Human Pancreatic Cancer Cell Line |
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| Authors: | Xinli Wang Guoqiang Chen Zhiyun Zhao Xiaodong Wang Zhili Li |
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| Affiliation: | Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing 100005, China |
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| Abstract: | To develop an economical and feasible approach to probe protein complexes, differential centrifugation and three‐dimensional polyacrylamide gel electrophoresis (PAGE) were performed to separate protein complexes from the cell lysate of human pancreatic cancer cell line, SW1990, followed by mass spectrometric identification. Four macromolecular protein complexes were separated and identified unambiguously. |
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| Keywords: | three dimensional polyacrylamide gel electrophoresis protein complex proteasome pancreatic cancer cell line mass spectrometry |
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