Simultaneous determination of five active hydrolysis ingredients from Panax quinquefolium L. by HPLC‐ELSD |
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| Authors: | Xiangrong Zhang Xiaoning Ma Bingkun Si Yuqing Zhao |
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| Institution: | 1. Shenyang Pharmaceutical University, Shenyang 110016, People's Republic of China;2. Liaoning University of Traditional Chinese Medicine, Shenyang 10032, People's Republic of China;3. Hangzhou Sharply Pharmaceutical R&D Institute Co. Ltd, Hangzhou 310052, People's Republic of China;4. Key Laboratory of Structure‐Based Drug Design, Discovery (Shenyang Pharmaceutical University), Ministry of Education, Shenyang 110016, People's Republic of China |
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| Abstract: | An effective method for simultaneous determination of five hydrolysis products of 20 (R)‐dammarane‐3β,6α,12β,20,25‐pentol, 24(R)‐ocotillol, 20(R)‐protopanaxatriol, 20(S)‐panaxatriol and 20(R)‐dammarane‐3β,12β,20,25‐tetrol was developed using high‐performance liquid chromatography with evaporative light scattering detection (HPLC‐ELSD). The hydrolysis products from Panax quinquefolium L. in the stems and leaves, berries, flower buds and roots components were successfully separated on a Kromasil C18 column using methanol and water (83:17, v/v) as mobile phase in 18 min. The parameter for the ELSD was set to a probe temperature of 40°C and the nebulizer for nitrogen gas was adjusted to 3 L/min. All calibration curves showed good linear regression (r > 0.9975) within test ranges. The validation of the method included recovery, linearity, accuracy and precision (intra‐ and inter‐day variation). The accuracy and precision were satisfactory, with the overall intra‐ and inter‐day variation being less than 3.11%, and recoveries of this method were greater than 95.0%. This study developed an effective and rapid method for simultaneous determination of multiple hydrolysis components from Panax quinquefolium L. Copyright © 2010 John Wiley & Sons, Ltd. |
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| Keywords: | HPLC‐ELSD hydrolysis products Panax quinquefolium L |
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