A modified protocol for isolation and purity evaluation of a staphylococcal acidic polysaccharide by chromatography and capillary electrophoresis |
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| Authors: | Maria I Krevvata Nikos Afratis Anastasia Spiliopoulou Christina J Malavaki Fevronia Kolonitsiou Evangelos Anastassiou Nikos K Karamanos |
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| Institution: | 1. Laboratory of Microbiology, School of Medicine, University of Patras, 265 00 Patras, Greece;2. Laboratory of Biochemistry, Section of Organic Chemistry, Biochemistry and Natural Products, Department of Chemistry, University of Patras, 265 00 Patras, Greece |
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| Abstract: | The extracellular slime of Staphylococcus epidermidis contains, amongst various macromolecules, an acidic polysaccharide (PS) of a molecular mass of 20 kDa with significant antigenic and biological properties. The isolation procedure used so far includes multiple fractionations in anion‐exchange chromatographic columns before its final purification by gel filtration chromatography. This protocol is laborious, time‐consuming and includes the risk of unnecessary loss of PS quantities. Because of the significance of this PS, a modified protocol resulting in an easier and quicker isolation procedure was developed. Furthermore, identification, purity, charge density and molecular integity of the isolated polysaccharide were evaluated by a reverse‐polarity capillary electrophoresis method. Copyright © 2010 John Wiley & Sons, Ltd. |
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| Keywords: | polysaccharide isolation capillary electrophoresis chromatography Staphylococcus |
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