Two‐Photon Probes for Zn2+ Ions with Various Dissociation Constants. Detection of Zn2+ Ions in Live Cells and Tissues by Two‐Photon Microscopy |
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Authors: | Dr. Yu Shun Tian Ji Hee Han Min Young Kang Prof. Dr. Bong Rae Cho |
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Affiliation: | Department of Chemistry, Research Institute for Natural Sciences, Korea University, 1‐Anamdong, Seoul 136‐701 (Korea), Fax: (+82)?2‐3290‐3121 |
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Abstract: | A series of Zn2+‐selective two‐photon fluorescent probes (AZnM1−AZnN) that had a wide range of dissociation constants (KdTP=8 nm‐ 12 μM ) were synthesized. These probes showed appreciable water solubility (>3 μM ), cell permeability, high photostability, pH insensitivity at pH>7, significant two‐photon action cross‐sections (86–110 GM) upon complexation with Zn2+, and can detect the Zn2+ ions in HeLa cells and in living tissue slices of rat hippocampal at a depth of >80 μm without mistargeting and photobleaching problems. These probes can potentially find application in the detection of various amounts of Zn2+ ions in live cells and intact tissues. |
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Keywords: | fluorescence fluorescent probes live tissue two‐photon microscopy zinc |
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