Application of capillary electrophoretic immunoassay to analysis of estradiol in women's serum |
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| Authors: | Ping Su Xin-Xiang Zhang Wen-Bao Chang |
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| Institution: | (1) Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education Department of Chemical Biology, College of Chemistry, Peking University, 100871 Beijing, China |
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| Abstract: | Summary A rapid and sensitive capillary electrophoretic immunoassay is described for determination of estradiol in women's serum.
Addition of thermally reversible hydrogel in the buffer, serving as a replaceable packing material, improved the reproducibility
of the method. Using a laser-induced fluorescence detector this method can be applied to the determination of estradiol at
concentrations as low as 30.6 pg mL1. Estradiol levels in 16 normal women's serum were measured at the range 115≈370 pg mL1. The results of this method have been found to correlate well with those of chemiluminescent immunoassay. |
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| Keywords: | Capillary electrophoretic immunoassay Laser-induced fluorescence Hydrogel Estradiol in Serum |
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