Theoretical model and quantification of reflectance photometer

The surface morphology of lateral flow (LF) strip is examined by scanning electron microscope (SEM) and the diffuse reflection of porous strip with or without nanogold particles is investigated.Based on the scattering and absorption of nanogold particles, a reflectance photometer is developed for quantification of LF strip with nanogold particles as reporter.The integration of reflection optical density is to indicate the signals of test line and control line.As an example, serial dilutions of microalbunminuria (MAU) solution are used to calibrate the performance of the reflectance photometer.The dose response curve is fitted with a four-parameter logistic mathematical model for the determination of an unknown MAU concentration.The response curve spans a dynamic range of 5 to 200 μg/ml.The developed reflectance photometer can realize simple and quantitative detection of analyte on nanogold-labeled LF strip.