Multiple peak formation of avidin on an iminobiotin affinity support |
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| Authors: | Wang Yan Li Tingyu |
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| Institution: | Department of Chemistry, Box 9573, Mississippi State University, Mississippi State, MS 39762, USA. TL45@ra.msstate.edu |
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| Abstract: | When pure avidin is chromatographed on a homemade iminobiotin column, two distinct peaks are observed using a gradient solvent system starting with a pH 9 buffer. Proteins in both peaks are shown to be identical by nondenatured polyacrylamide gel electrophoretic experiment. The weak interaction of the protein with the stationary phase at pH 9 could cause this unusual behavior. |
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