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Studies on elution conditions for the determination of anions by supressed ion-interaction chromatography using a graphitized carbon column |
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| Authors: | Toshimitsu Okamoto Akinori Isozaki Hisomu Nagashima |
| Institution: | a Laboratory of Analytical Science, Product Development Laboratories, Sankyo Co., Ltd., 1-2-58, Hiromachi, Shinagawa-ku, Tokyo 140, Japan b Department of Industrial Chemistry, College of Science and Technology, Nihon University, 1-8-14, Kanda-Surugadai, Chiyoda-ku, Tokyo 101, Japan c Institute of Science and Technology, Inc., 3-10-2, Kitashinagawa, Shinagawa-ku, Tokyo 140, Japan |
| Abstract: | A new method has been developed for ion-interaction chromatography with suppressed conductivity detection and a new graphitized carbon packing, which is sintered from carbonic material at a high temperature. Combinations of various eluting agents, tetrabutylammonium hydroxide (TBA) and acetonitrile have been investigated to optimize the separation of eight common anions (F?, Cl?, NO2?, Br?, NO3?, SO42?, HPO42? and I?). Calibration curves were linear from 0.5 to 10 μg/ml for F?, from 1.0 to 20 μg/ml for Cl?, NO2? and NO3?, from 2.5 to 50 μg/ml for Br? and SO42? and from 5.0 to 100 μg/ml for HPO42? and I? with a correlation coefficient (r) of 0.999 or better. The relative standard deviations (R.S.D.s) of peak areas were between 0.2 and 0.9% for 10 repeated measurements. The application of this newly developed method was demonstrated by the determination of chloride, bromide and sulfate in pharmaceutical compounds using the direct injection method. The analytical results were within ±2% (relative) of the theoretical value, and thus in good agreement with the theoretical value for each sample. |
| Keywords: | Anions Tetrabutylammonium |
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