A novel HPQ-based turn-on fluorescent probe for detection of fluoride ions in living cells |
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| Authors: | Zhou Zhao Xinzhou Bi Wuxiang Mao Xiaowei Xu |
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| Institution: | 1. State Key Laboratory of Natural Medicines, Key Lab of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, 210009 Nanjing, China;2. State Key Laboratory of Natural Medicines, Center of Drug Discovery, China Pharmaceutical University, 210009 Nanjing, China;3. Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, College of Life Sciences, Hubei University, Wuhan, Hubei 430062, China |
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| Abstract: | 2-(2′-Hydroxyphenyl)-4(3H)-quinazolinone (HPQ) has been reported as a precipitating fluorescent molecule with excellent optical properties, such as large Stokes shift and strong fluorescence intensity. HPQF, a novel HPQ-based turn-on probe for localizable detection of fluoride ions, was designed, synthesized and fully characterized by 1H NMR, 13C NMR and HRMS. As a chemogenic fluoride probe, the tert-butyldiphenylsilane moiety of HPQF can be easily cleaved by fluoride. After spontaneous 1,6-elimination, HPQ molecule was generated to emit fluorescence under the excitation light. Further study shows that HPQF exhibited high selectivity and sensitivity for detection of fluoride. In addition, HPQF was utilized for the detection of fluoride in living cells. |
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| Keywords: | HPQ Fluoride Fluorescence probe |
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