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| 牛血清白蛋白手性毛细管整体柱的制备及对映体的分离 | |
| 引用本文： | 严丽娟,张庆合,张维冰,冯钰锜,张丽华,李彤,张玉奎. 牛血清白蛋白手性毛细管整体柱的制备及对映体的分离[J]. 高等学校化学学报, 2005, 26(5): 835-837 |
| 作者姓名： | 严丽娟 张庆合 张维冰 冯钰锜 张丽华 李彤 张玉奎 |
| 作者单位： | 中国科学院大连化学物理研究所,国家色谱研究分析中心,大连,116023;武汉大学化学与分子科学学院,武汉,430072;中国科学院大连化学物理研究所,国家色谱研究分析中心,大连,116023;大连依利特分析仪器有限公司,大连,116011;中国科学院大连化学物理研究所,国家色谱研究分析中心,大连,116023;武汉大学化学与分子科学学院,武汉,430072 |
| 摘 要： | A silica-bonded bovine serum albumin (BSA) chiral monolithic stationary phase for capillary electrochromatography(CEC) was introduced. An inorganic-organic hybrid monolithic column was firstly prepared by sol-gel chemistry with homogeneously distributed aminopropyl groups throughout the silica matrix. Then the chiral stationary phase was synthesized by the in situ covalent immobilization of BSA on the monolithic column activated with glutaraldehyde. The effects of pH value and concentration of phosphate buffer on the separation of D,L-tryptophan were investigated. The separation factor of D,L-tryptophan reached 3.37 on CEC mode. |
| 关 键 词： | 毛细管电色谱 整体柱 溶胶-凝胶法 手性固定相 牛血清白蛋白 |
| 文章编号： | 0251-0790(2005)05-0835-03 |
| 收稿时间： | 2004-11-10 |
Preparation of Silica-bonded Bovine Serum Albumin Chiral Monolithic Column for Enantiomeric Separation by Capillary Electrochromatography |
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| YAN Li-juan,ZHANG Qing-He,ZHANG Wei-Bing,FENG Yu-qi,ZHANG Li-Hua,LI Tong,ZHANG Yu-kui. Preparation of Silica-bonded Bovine Serum Albumin Chiral Monolithic Column for Enantiomeric Separation by Capillary Electrochromatography[J]. Chemical Research In Chinese Universities, 2005, 26(5): 835-837 | |
| Authors: | YAN Li-juan ZHANG Qing-He ZHANG Wei-Bing FENG Yu-qi ZHANG Li-Hua LI Tong ZHANG Yu-kui |
| Affiliation: | 1. National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China; 2. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China; 3. Dalian Elite Analytical Instruments Co. Ltd., Dalian 116011, China |
| Abstract: | A silica-bonded bovine serum albumin(BSA) chiral monolithic stationary phase for capillary electrochromatography(CEC) was introduced. An inorganic-organic hybrid monolithic column was firstly prepared by sol-gel chemistry with homogeneously distributed aminopropyl groups throughout the silica matrix. Then the chiral stationary phase was synthesized by the in situ covalent immobilization of BSA on the monolithic column activated with glutaraldehyde. The effects of pH value and concentration of phosphate buffer on the separation of D,L-tryptophan were investigated. The separation factor of D,L-tryptophan reached 3.37 on CEC mode. |
| Keywords: | Capillary electrochromatography(CEC) Monolithic column Sol-gel Chiral stationary phases(CSPs) Bovine serum albumin(BSA) |
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