气相色谱-质谱联用结合化学计量学方法分析光照对鱼腥草挥发性成分的影响

采用色谱-质谱联用技术结合化学计量学方法对不同光照生长条件的鱼腥草挥发性成分进行分析。水蒸气蒸馏法提取鱼腥草挥发油，气相色谱-质谱进行分析。色谱指纹图谱结合主成分分析与相似度评价以探究不同光照挥发油成分的异同。直观推导式演进特征投影法(Heuristic evolving latent projections,HELP)分辨重叠色谱峰,NIST标准质谱库结合相关文献进行定性，峰面积归一化法定量，t-检验比较不同光照组成分含量间是否有显著性差异。3种光照下的鱼腥草挥发油指纹图谱存在一定的共性和差异，共鉴定出33种化合物，共有化合物26种。随着光照强度的减少，单萜类化合物含量减少，非萜类含量增多，倍半萜类变化不大。癸酰乙醛和甲基正壬酮在全光照组的含量明显低于遮光组的含量。研究结果表明，适度的遮光有利于鱼腥草挥发性有效成分含量的提高，鱼腥草药材挥发性成分种类及含量与生长条件密切相关。

Analysis of Effect of Light Intensity on the Volatile Components in Houttuynia cordata Thunb by Gas Chromatography-Mass Spectrometry Combined with Chemometric Methods

The aim of this research is to provide a scientific basis for quality control of Houttuynia cordata Thunb through the analysis of the volatile components under different light intensities by gas chromatograph-mass spectrometry(GC-MS) combined with chemometric methods.The volatile oils were extracted by steam distillation and analyzed by GC-MS.The similarities and differences among volatiles under different light intensities were analyzed by chromatographic fingerprint,principle component analysis and similarity evaluation.The overlapping chromatographic peaks were deconvoluted by heuristic evolving latent projections(HELP) method.The qualitative and quantitative analysis were performed by NIST standard mass spectral library combined with relevant literature and peak area normalization method.T-test was used to compare the differences of volatile constituent contents among different groups.There were some similarities and differences in volatile oil fingerprint among 3 groups under different light intensities.Totally,33 compounds were identified in the volatile oil from 3 groups,of which 26 components were common to all materials.The content of monoterpenoids had the positive correlation with light intensity,and the content of non-terpenoids had the negative correlation with light intensity.The content of sesquiterpenes had no relation with light intensity.The t-test results indicated that there was significant difference in the content of some common compositions.The content of decanoyl acetaldehyde and 2-undecanone in group with full light were significantly lower than the other two groups with shading.The results suggested that moderate shading was beneficial to the improvement of content of effective components in Houttuynia cordata Thunb volatile.The type and content of volatile components in Houttuynia cordata Thunb were closely related to the growth conditions.