Development and Validation an LC Method for the Determination of Bendroflumethiazide in Human Plasma and its Pharmacokinetics

A simple, rapid, sensitive high performance liquid chromatography method with fluorescent detection was developed and validated for the determination of bendroflumethiazide in human plasma. Extraction from the plasma was by liquid-liquid extraction using ethyl acetate. Mosapride citrate was used as the internal standard. The chromatographic separation was performed on reverse phase LiChrosphere C18 column with mobile phase comprising of acetonitrile and phosphate buffer (38:62 v/v). The assay precision ranged from 0.9–12.5 and accuracy between 96.8–108.8%, revealing that the method has good reproducibility over the concentration range of 0.98–100.16 ng mL⁻¹. The validated method has been applied to analyze the bendroflumethiazide concentrations for application in pharmacokinetic, bioavailability or bioequivalence studies.