| 一种蛋白质阵列芯片新技术的研究 |
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| 引用本文： | 韩欢欢,于晓波,吕任极,何为,许丹科. 一种蛋白质阵列芯片新技术的研究[J]. 分析试验室, 2008, 27(2): 107-110 |
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| 作者姓名： | 韩欢欢 于晓波 吕任极 何为 许丹科 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所,北京,100850;北京蛋白质组研究中心,北京,102206;军事医学科学院放射与辐射医学研究所,北京,100850;北京蛋白质组研究中心,北京,102206 |
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| 摘 要： | 将微印刷技术与银增强法相结合,建立了一种蛋白质阵列芯片制备与检测的新方法。通过定量分析证明了蛋白质可以被均匀地转印至固相载体表面,采用银增强法对蛋白质的直接法检测和夹心法检测的最低检测量分别可达0.33和0.13 fmol。此新方法应用于蛋白质阵列上具有灵活、灵敏及成本低廉等特点。
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| 关 键 词： | 蛋白阵列芯片 微印刷技术 银增强检测 |
| 文章编号： | 1000-0720（2008）02-107-04 |
| 收稿时间： | 2006-12-11 |
| 修稿时间： | 2007-02-20 |
A novel technique for protein arrays based on a microcontact printing method coupled with silver enhancement detection |
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| HAN Huan-huan,YU Xiao-bo,L Ren-ji,HE Wei,XU Dan-ke. A novel technique for protein arrays based on a microcontact printing method coupled with silver enhancement detection[J]. Chinese Journal of Analysis Laboratory, 2008, 27(2): 107-110 |
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| Authors: | HAN Huan-huan YU Xiao-bo L Ren-ji HE Wei XU Dan-ke |
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| Affiliation: | HAN Huan-huan,YU Xiao-bo,L(U) Ren-ji,HE Wei,XU Dan-ke |
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| Abstract: | In this report,a microcontact printing technique coupled with silver enhancement method was developed to fabricate and assay for protein arrays.It has been demonstrated through quantitative measurement of protein arrays that proteins could be transferred to the slide homogeneously.The detection limits of 0.33 fmol and 0.13 fmol for direct and sandwich detection were obtained respectively.The present method showed its several advantages such as its flexibility,sensitivity and cost-effectivity. |
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| Keywords: | Protein array Microcontact printing Silver enhancement method |
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