NMR Fingerprints of the Drug‐like Natural‐Product Space Identify Iotrochotazine A: A Chemical Probe to Study Parkinson’s Disease |
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| Authors: | Dr Tanja Grkovic Dr Rebecca H Pouwer Marie‐Laure Vial Luca Gambini Alba Noël Dr John N A Hooper Dr Stephen A Wood Dr George D Mellick Prof?Dr Ronald J Quinn |
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| Institution: | Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD, 4111 (Australia) |
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| Abstract: | The NMR spectrum of a mixture of small molecules is a fingerprint of all of its components. Herein, we present an NMR fingerprint method that takes advantage of the fact that fractions contain simplified NMR profiles, with minimal signal overlap, to allow the identification of unique spectral patterns. The approach is exemplified in the identification of a novel natural product, iotrochotazine A ( 1 ), sourced from an Australian marine sponge Iotrochota sp. Compound 1 was used as a chemical probe in a phenotypic assay panel based on human olfactory neurosphere‐derived cells (hONS) from idiopathic Parkinson’s disease patients. Compound 1 at 1 μM was not cytotoxic but specifically affected the morphology and cellular distribution of lysosomes and early endosomes. |
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| Keywords: | natural products NMR spectroscopy NMR fingerprints Parkinson’ s disease total synthesis |
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