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Acidic‐pH‐Activatable Fluorescence Probes for Visualizing Exocytosis Dynamics |
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| Authors: | Shigeyuki Namiki Kenji Takikawa Mako Kamiya Prof. Tetsuo Nagano Prof. Yasuteru Urano Prof. Kenzo Hirose |
| Affiliation: | 1. Graduate School of Medicine, The University of Tokyo, 7‐3‐1 Hongo, Bunkyo‐ku, Tokyo (Japan);2. Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7‐3‐1 Hongo, Bunkyo‐ku, Tokyo (Japan);3. Open Innovation Center for Drug Discovery, The University of Tokyo, 7‐3‐1 Hongo, Bunkyo‐ku, Tokyo (Japan) |
| Abstract: | Live imaging of exocytosis dynamics is crucial for a precise spatiotemporal understanding of secretion phenomena, but current approaches have serious limitations. We designed and synthesized small‐molecular fluorescent probes that were chemically optimized for sensing acidic intravesicular pH values, and established that they can be used to sensitively and reliably visualize vesicular dynamics following stimulation. This straightforward technique for the visualization of exocytosis as well as endocytosis/reacidification processes with high spatiotemporal precision is expected to be a powerful tool for investigating dynamic cellular phenomena involving changes in the pH value. |
| Keywords: | acidity exocytosis fluorescent probes spatiotemporal dynamics vesicular imaging |