Building Proficient Enzymes with Foldamer Prostheses |
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Authors: | Clemens Mayer Dr. Manuel M. Müller Prof. Dr. Samuel H. Gellman Prof. Dr. Donald Hilvert |
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Affiliation: | 1. Laboratory of Organic Chemistry, ETH Zürich, H?nggerberg HCI F339, 8093 Zürich (Switzerland);2. Department of Chemistry, University of Wisconsin Madison, 1101 University Avenue, Madison, WI 53706 (USA) |
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Abstract: | Foldamers are non‐natural oligomers that adopt stable conformations reminiscent of those found in proteins. To evaluate the potential of foldameric subunits for catalysis, semisynthetic enzymes containing foldamer fragments constructed from α‐ and β‐amino acid residues were designed and characterized. Systematic variation of the α→β substitution pattern and types of β‐residue afforded highly proficient hybrid catalysts, thus demonstrating the feasibility of expanding the enzyme‐engineering toolkit with non‐natural backbones. |
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Keywords: | β ‐amino acids enzymes foldamers protein design protein engineering |
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