| 毛细管电泳——多光子激发荧光检测分析生物胺 |
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| 引用本文： | 张桂森,陈胜,徐友志,杜伟,刘笔锋. 毛细管电泳——多光子激发荧光检测分析生物胺[J]. 光子学报, 2008, 37(5): 1006-1009 |
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| 作者姓名： | 张桂森 陈胜 徐友志 杜伟 刘笔锋 |
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| 作者单位： | 华中科技大学,生物医学光子学教育部重点实验室,武汉,430074;华中科技大学,生物医学光子学教育部重点实验室,武汉,430074;湖北师范学院,电工电子教学示范中心,湖北,黄石,435001 |
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| 基金项目： | 国家自然科学基金 , 华中科技大学校科研和教改项目 |
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| 摘 要： | 构建了毛细管电泳－连续光多光子激发荧光检测系统并应用于生物分析.对几种常见生物胺,如腐胺,尸胺,组胺,精胺,亚精胺和苯乙胺的分析结果表明,该系统具有分离效率高、质量检测灵敏度高和低消耗等特点,适于复杂体系如生物样品的测量.定量分析显示,生物胺的检测限在nM级,线性范围超过2个数量级,进一步将多光子激发荧光检测与传统单光子激发荧光检测结果对比,发现前者还具有改善分离选择性的优势.
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| 关 键 词： | 毛细管电泳 多光子激发荧光 生物胺 |
| 收稿时间： | 2006-10-26 |
| 修稿时间： | 2007-03-20 |
Determination of Biogenic Amines by Capillary Electrophoresis Coupling with Continuous Wave-based Multiphoton Excited Fluorescence Detection |
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| ZHANG Gui-sen,CHEN Sheng,XU You-zhi,DU Wei,Liu Bi-Feng. Determination of Biogenic Amines by Capillary Electrophoresis Coupling with Continuous Wave-based Multiphoton Excited Fluorescence Detection[J]. Acta Photonica Sinica, 2008, 37(5): 1006-1009 |
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| Authors: | ZHANG Gui-sen CHEN Sheng XU You-zhi DU Wei Liu Bi-Feng |
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| Abstract: | Multiphoton excited fluorescence (MPEF) detection coupling to capillary electrophoresis (CE) has been proposed to analyze biogenic amines,such as putrescine,cadaverine,histamine,spermine,spermidine and phenylethylamine.The detection limit was found to be nM-level,with linear range of over two orders of magnitude for quantitation.It showed that the constructed CE-MPEF method was well suited for complex systems like bio-sample,with advantages of high separation performance,high detection sensitivity and low cost.Further,MPEF exhibited a capability for improving separation resolution after comparing with conventional single photon laser induced fluorescence. |
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| Keywords: | Capillary electrophoresis Multiphoton excitation Biogenic amines |
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