Use of thiosulfonate for the protection of thiol groups in peptide ligation by the thioester method |
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Authors: | Takeshi SatoSaburo Aimoto |
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Affiliation: | Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan |
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Abstract: | Use of thiosulfonate for protecting thiol (-SH) groups in peptide ligation by the thioester method was examined. Thiosulfonate was introduced and was stable in the presence of silver ion, 4-dihydro-3-hydroxy-4-oxo-1,2,3-benzotriazine, and diisopropylethylamine. Based on these results, a strategy for using the thioester method and the native chemical ligation method in the synthesis of a single polypeptide is described. |
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