AlkB recognition of a bulky DNA base adduct stabilized by chemical cross-linking |
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| Authors: | YI ChengQi & HE Chuan |
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| Affiliation: | YI ChengQi1 & HE Chuan1,2 1Department of Chemistry,University of Chicago,Chicago,Illinois 60637,USA,2Department of Chemical Biology,College of Chemistry , Molecular Engineering,Peking University,Beijing 100871,China |
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| Abstract: | E.coli AlkB is a direct DNA/RNA repair protein that oxidatively reverses N1 alkylated purines and N3 alkylated pyrimidines to regular bases.Previous crystal structures have revealed N1-methyl adenine(1-meA) recognition by AlkB and a unique base flipping mechanism,but how the AlkB active site can accommodate bulky base adducts is largely unknown.Employing a previously developed chemical cross-linking technique,we crystallized AlkB with a duplex DNA containing a caged thymine base(cagedT).The structure reveal... |
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| Keywords: | DNA/RNA repair AlkB caged thymine disulfide cross-linking bulky base adduct |
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