| Imaging Array SPR Biosensor Immunoassays for Sulfamethoxazole and Sulfamethazine |
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| 作者姓名： | Hui LI Da Fu CUI Jin Qing LIANG HaoYuan CAI Yu Jie WANG |
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| 作者单位： | Hui LI，Da Fu CUI，Jin Qing LIANG，HaoYuan CAI，Yu Jie WANG State Key Laboratory of Transducer Technology，Institute of Electronics，Chinese Academy of Sciences，Beijing 100080 |
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| 基金项目： | 国家自然科学基金;国家高技术研究发展计划(863计划) |
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| 摘 要： | A homemade array surface plasmon resonance (SPR)-based imaging biosensor was used to develop sensitive and fast immunoassays to determine sulfamethoxazole (SMOZ) and sulfamethazine (SMT) in buffer. Two conjugations of sulfonamide-bovine serum albumin (BSA) were separately immobilized on two different rows of the array chip with one row as reference. The immobilization was carried out in the instrument to monitor the quantity of the conjugations immobilized. The antibody mixed with the sulfonamide in the buffer was injected over the surface of the chip to get a relative response which was inversely proportional to the concentration of the sulfonamide in the PBS buffer. Two calibration curves were constructed and the limit of detection for sufamethoxazole in buffer was 3.5 ng/mL and for sulfamethazine 0.6 ng/mL. The stability and specificity of the antibody were also studied. The monoclonal antibody did not bind with BSA.
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| 关 键 词： | 成像 表面细胞质基因 生物传感器 磺胺甲嘧啶 |
| 收稿时间： | 2006-08-30 |
Imaging Array SPR Biosensor Immunoassays for Sulfamethoxazole and Sulfamethazine |
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| Hui LI Da Fu CUI Jin Qing LIANG HaoYuan CAI Yu Jie WANG.Imaging Array SPR Biosensor Immunoassays for Sulfamethoxazole and Sulfamethazine[J].Chinese Chemical Letters,2006,17(11):1481-1484. |
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| Authors: | Hui LI Da Fu CUI Jin Qing LIANG HaoYuan CAI Yu Jie WANG |
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| Abstract: | A homemade array surface plasmon resonance (SPR)-based imaging biosensor was used to develop sensitive and fast immunoassays to determine sulfamethoxazole (SMOZ) and sulfamethazine (SMT) in buffer. Two conjugations of sulfonamide-bovine serum albumin (BSA) were separately immobilized on two different rows of the array chip with one row as reference. The immobilization was carried out in the instrument to monitor the quantity of the conjugations immobilized. The antibody mixed with the sulfonamide in the buffer was injected over the surface of the chip to get a relative response which was inversely proportional to the concentration of the sulfonamide in the PBS buffer. Two calibration curves were constructed and the limit of detection for sufamethoxazole in buffer was 3.5 ng/mL and for sulfamethazine 0.6 ng/mL. The stability and specificity of the antibody were also studied. The monoclonal antibody did not bind with BSA. |
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| Keywords: | Imaging surface plasmon resonance biosensor reference surface sulfamethoxazole sulfamethazine |
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