Direct determination of non-steroidal anti-inflammatory drugs by column-switching LC-MS |
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Authors: | Suenami Koichi Wah Lim Lee Takeuchi Toyohide Sasajima Yasuhide Sato Kiyohito Takekoshi Yuji Kanno Susumu |
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Affiliation: | Department of Chemistry, Faculty of Engineering, Gifu University, Gifu, Japan. c18871@pref.gifu.lg.jp |
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Abstract: | A method for determination of 16 non-steroidal anti-inflammatory drugs (NSAIDs) in human plasma samples without time-consuming sample pre-treatments was developed. The system consisted of two pumps for mobile phase delivery, a six-port switching valve, a pre-column (Oasis HLB Cartridge Column), and a reversed phase analytical column (COSMOSIL 3C18-MS-II). The analytes were trapped on the precolumn and subsequently separated on the analytical column. The present method allowed on-line sample clean-up and enrichment, leading to improved sensitivity without any tedious sample preparation. The recoveries of NSAIDs from human plasma by column-switching were greater than 72.6%. The total analysis time for a single analytical run was approximately 11 min. The detection limits of NSAIDs were 0.0025 to 0.2 microg/mL using the selected ion monitoring mode. |
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Keywords: | Column‐switching Human plasma LC–MS Non‐steroidal anti‐inflammatory drugs |
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