CONFIGURATIONS OF CAROTENOIDS IN THE REACTION CENTER and THE LIGHT-HARVESTING COMPLEX OF Rhodospirillum rubrum. NATURAL SELECTION OF CAROTENOID CONFIGURATIONS BY PIGMENT PROTEIN COMPLEXES |
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Authors: | Yasushi Koyama Ichiro Takatsuka Mariko Kanaji Koichi Tomimoto Mariko Kito Toshio Shimamura Jinpei Yamashita Kayoko Saiki Kiyoshi Tsukida |
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Institution: | Faculty of Science, Kwansei Gakuin University, Uegahara, Nishinomiya 662, Japan;Nitto Technical Information Center, Shimo-Hozumi, Ibaraki 567, Japan;Radioisotope Research Centre, Osaka University, Suita, Osaka 565, Japan;Kobe Women's College of Pharmacy, Motoyama-Kitamachi, Kobe 658, Japan |
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Abstract: | Abstract— Carotenoids extracted from the reaction center (RC), the light-harvesting complex (LH), and the chromatophore membrane of Rhodospirillum rubrum SI were analyzed by high-performance liquid chromatography. The chemical structures and the configurations of major components were determined by means of mass, Raman, electronic absorption and 1H-NMR spectroscopy. The results indicated: (1) 15- cis -spirilloxanthin is bound to RC; (2) both all-frans-spirilloxanthin and aII-(ran.s-3,4-dihydrospirilloxanthin are bound to LH and (3) 13-cK-spirilloxanthin is additionally present in the chromatophore membrane. The natural selection of the carotenoid configurations, i.e. 15-ris by RC and aW-trans by LH, is discussed in relation to the physiological functions and the photophysical properties of isomeric carotenoids. |
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