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Development of novel magnetic nano-carriers for high-performance affinity purification
Authors:Nishio Kosuke  Masaike Yuka  Ikeda Morihito  Narimatsu Hiroki  Gokon Nobuyuki  Tsubouchi Shingo  Hatakeyama Mamoru  Sakamoto Satoshi  Hanyu Naohiro  Sandhu Adarsh  Kawaguchi Haruma  Abe Masanori  Handa Hiroshi
Institution:Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, Japan.
Abstract:We developed novel magnetic nano-carriers around 180 nm in diameter for affinity purification. Prepared magnetic nano-carriers possessed uniform core/shell/shell nano-structure composed of 40 nm magnetite particles/poly(styrene-co-glycidyl methacrylate (GMA))/polyGMA, which was constructed by admicellar polymerization. By utilizing relatively large 40 nm magnetite particles with large magnetization, the magnetic nano-carriers could show good response to permanent magnet. Thanks to uniform polymer shell with high physical/chemical stability, the magnetic nano-carriers could disperse in a wide range of organic solvent without disruption of core/shell structure and could immobilize various kinds of drugs. We examined affinity purification using our prepared magnetic nano-carriers with anti-cancer agent methotrexate (MTX) as ligand. Our magnetic nano-carriers showed higher performance compared to commercially available magnetic beads in terms of purification efficiency of target including extent of non-specific binding protein.
Keywords:Magnetite  Polymerization  Magnetic carrier  Affinity purification  Drug target
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