Aptamer-quantum dots conjugates-based ultrasensitive competitive electrochemical cytosensor for the detection of tumor cell |
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Authors: | Li Jingjing Xu Mi Huang Haiping Zhou Jinjun Abdel-Halimb E S Zhang Jian-Rong Zhu Jun-Jie |
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Institution: | a State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, People's Republic of China b Petrochemical Research Chair, Chemistry Department, College of Science, King Saud University, Riyadh 11451, P.O. Box 2455, Kingdom Saudi Arabia c Center for Materials Analysis and Testing, Jiangxi University of Science and Technology, Ganzhou, 341000, People's Republic of China |
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Abstract: | A novel competitive electrochemical cytosensor was reported by using aptamer (Apt)-quantum dots (Qdots) conjugates as a platform for tumor cell recognition and detection. The complementary DNA (cDNA), aptamer and Qdots could be assembled to the gold electrode surface. When the target cells existed, they could compete with cDNA to bind with Apt-Qdots conjugates based on the specific recognition of aptamer to MUC1 protein overexpressed on the cell surface, which resulted in the denaturation of double-stranded DNA structure and the release of the Apt-Qdots conjugates from the electrode. Electrochemical stripping measurement was then employed to determine the Cd2+ concentration in Qdots left at the electrode. The peak current was inversely proportional to the logarithmic value of cell concentration ranging from 1.0 × 102 to 1.0 × 106 cells mL−1 with a detection limit of 100 cells mL−1. Meanwhile, the recognition of aptamer to the target cells could be clearly observed through the strong fluorescence from Qdots. This is an example of the combination of aptamer and nanoparticles for the application of cell analysis, which is essential to cancer diagnosis and therapy. |
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Keywords: | Aptamer Cancer cell Electrochemistry Fluorescence imaging Quantum dots |
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