Spectrophotometric detection of tyrosinase activity based on boronic acid-functionalized gold nanoparticles |
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| Authors: | Li Sa Mao Langyong Tian Yaping Wang Joseph Zhou Nandi |
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| Affiliation: | The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China. |
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| Abstract: | A spectrophotometric method for the detection of tyrosinase activity is developed by utilizing the product-triggered aggregation of boronic acid-functionalized gold nanoparticles. Based on the changes of absorbance in UV-visible spectra, the assay shows extremely high sensitivity and lowered limit of detection of 1 × 10(-10) u mL(-1). |
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