胰腺癌和慢性胰腺炎的胰液核磁共振1H谱分析

运用核磁共振波谱技术对临床慢性胰腺炎和胰腺癌患者的胰液样品进行体外波谱学分析，观察和鉴别胰腺癌和慢性胰腺炎的特征代谢物变化，从而为胰腺癌以及相关胰腺疾病的病理研究提供基础数据和实验方法. 本文利用胰液样品在室温下能长时间稳定的特性，对其进行了质子的1D、TOCSY和J-resolved等多种NMR实验，并对其主要特征峰进行了指认. 实验结果显示在δ 1.19的三重峰是这两种病变在胰液中的主要区别：只在慢性胰腺炎图谱中观察到，而在胰腺癌图谱中未见. D实验结果表明此特征峰为乙氧基中的甲基峰，而乙氧基可能与胰腺中乙醇的代谢有关

NMR Spectra of Pancreatic Fluids Can Be Used to Differentiate Chronic Pancreatitis from Pancreatic Cancer

We applied nuclear magnetic resonance spectroscopy（NMR）to study pancreatic juice (PJ) taken from patients with chronic pancreatitis (CP) and pancreatic cancer. The aim of the study is to assign the main resonances in the ¹H NMR spectra of PJ and to discover the characteristic metabolites the changes of which can be used to differentiate CP from pancreatic cancer. 1D proton spectroscopy, 2D TOCSY and J-resolved spectra (JRES) were performed to assign the main resonances. We found a triplet at δ1.19 that only appeared in the spectra of PJ from the CP patients, but not in the spectra of PJ from the pancreatic cancer patients. The triplet was assigned to be the resonance of ethoxyl, which might be associated with the metabolism of alcohol in pancreas.