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Determination of the postmortem interval by Laser Induced Breakdown Spectroscopy using swine skeletal muscles
Affiliation:1. Center for Transportation Research, Argonne National Laboratory, Lemont, IL, USA;2. Department of Mechanical, Materials, and Aerospace Engineering, Illinois Institute of Technology, Chicago, IL, USA;1. NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, 117456 Singapore, Singapore;2. Singapore Institute of Manufacturing Technology (SIMTech), 2 Fusionopolis Way, #08-04, 138634 Singapore, Singapore;3. Department of Materials Science and Engineering, National University of Singapore, 117576 Singapore, Singapore;1. College of Forensic Medicine, Hebei Medical University, Hebei Key Laboratory of Forensic Medicine, Collaborative Innovation Center of Forensic Medical Molecular Identification, Hebei, Shijiazhuang 050017, China;2. Institute of Forensic Medicine and Laboratory Medicine, Jining Medical University, Forensic Science Center of Jining Medical University, Shandong, Jining 272067, China
Abstract:Skin and muscle samples are useful to discriminate individuals as well as their postmortem interval (PMI) in crime scenes and natural or caused disasters. In this study, a simple and fast method based on Laser Induced Breakdown Spectroscopy (LIBS) has been developed to estimate PMI using swine skeletal muscle samples. Environmental conditions (moisture, temperature, fauna, etc.) having strong influence on the PMI determination were considered. Time-dependent changes in the emission intensity ratio for Mg, Na, Hα and K were observed, as a result of the variations in their concentration due to chemical reactions in tissues and were correlated with PMI. This relationship, which has not been reported previously in the forensic literature, offers a simple and potentially valuable means of estimating the PMI.
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