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A New Thiophenyl Pyrazoline Fluorescent Probe for Cu2+ in Aqueous Solution and Imaging in Live Cell |
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| Authors: | Meng-Meng Li Wen-Bo Zhao Ting-Ting Zhang Wei-Liu Fan Yu Xu Yu Xiao Jun-Ying Miao Bao-Xiang Zhao |
| Affiliation: | 1. School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, People’s Republic of China 2. School of Life Science, Shandong University, Jinan, 250100, People’s Republic of China |
| Abstract: | A new thiophenyl pyrazoline probe for Cu2+ in aqueous solution was synthesized and characterized by IR, NMR, HRMS and X-ray analysis. The probe displays remarkably high selectivity and sensitivity for Cu2+ with a detection limit of 1.919?×?10?7 M in aqueous solution (EtOH:HEPES = 1:1, v/v, 0.02 M, pH?=?7.2). In addition, the probe is further successfully used to image Cu2+ in living cells and the probe possesses good reversibility. A new thiophenyl pyrazoline probe can be used to image Cu2+ in living cells with good reversibility |
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