Highly sensitive differential pulse amperometric detection of second generation anti-tumor platinum compounds in HPLC effluents |
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Authors: | W J F van der Vijgh H B J van der Lee G J Postma H M Pinedo |
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Institution: | (1) Research Laboratory of Internal Medicine and Department of Oncology, Free University Hospital, De Boelelaan 1117, NL-1007 MB Amsterdam, The Netherlands |
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Abstract: | Summary A highly sensitive on-line amperometric detection of platinum compounds in HPLC effluents was possible with the use of a polarographic detector. For TNO-1 (=cis-1,1-di(aminomethyl)-cyclohexane Pt(II)chloride) a linear dynamic range of at least three decades could be obtained (0.034–108g TNO-1/ml) with a detection limit of three times the noise signal at 0.7 ng TNO-1 (20ng Pt/ml). The sensitivity was 0.1 nA/ng TNO-1 (0.2nA/ng Pt). The within-day presicion was 1.1% at a concentration of 10.8g TNO-1/ml (n=10). With this system the compatibility was measured of TNO-6 with infusion fluids. In 0.15 M NaCl, TNO-6 (=cis-1,1-di(aminomethyl)-cyclohexanePt(II)sulphate) was converted into TNO-1 within 40 minutes. Molecular changes of TNO-6 were also observed in a 5% aqueous glucose solution. |
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Keywords: | anti-tumor platinum compounds HPLC retention sensitive on-line detection differential pulse amperometry |
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