Principle and applications of the partial filling technique in capillary electrophoresis |
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Authors: | A Amini U Paulsen-Sörman D Westerlund |
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Institution: | (1) Analytical Pharmaceutical Chemistry, Uppsala University Biomedical Centre, Box 574, 751 23 Uppsala, Sweden;(2) Division of Chemistry & Pharmacy, Medical Products Agency, Box 26, 751 03 Uppsala, Sweden;(3) Present address: Department of Chemistry, Purdue University, 47907 Lafayette, IN, USA |
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Abstract: | Summary The partial filling technique (PFT) in capillary electrophoresis (CE) is an efficient system where, only 50–800 nanolitres
of a chiral selector solution needs to be added to each run. PFT is especially applicable when these additives to the background
electrolyte (BGE) are expensive or absorb UV light. The selector dissolved in the BGE is applied to the capillary as a plug,
shorter than the effective length of the capillary, prior to application of the analyte. During the run both ends of the capillary
are connected to the BGE. The applied plug and the analyte may move in opposite directions or in the same direction at different
velocities depending on their electrophoretic mobilities. Thus the final plug length is either longer or shorter than the
original length. The technique has been successfully applied in a number of studies including enantiomeric separation with
a variety of selectors, and for the determination of conditional association constants.
Taken from Dr. Pharm. Sc. Thesis, A. Amini, Uppsala, 1998. |
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Keywords: | Review Capillary electrophoresis Partial filling technique Selectivity and resolution Effective plug length |
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