|
|||||
|
|
[31P]NMR measurements of hexokinase activity in intact red blood cells with 2-deoxyglucose as substrate |
|
| Affiliation: | 1. Department of Chemistry, SRM Institute of Science and Technology, Chennai 603 203, India;2. Department of Chemical Sciences, Indian Institute of Science Education and Research, Mohali, Knowledge City, Sector 81, S. A. S. Nagar, Manauli PO, Mohali. 140306. Punjab. India;3. Organic Chemistry Division, CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411 008, India |
| Abstract: | [31P] NMR spectroscopy is demonstrated to be a suitable tool to follow the time course of 2-deoxyglucose-6-phosphate in intact human erythrocytes incubated with 2-deoxyglucose. It allowed to determine hexokinase Vmax and Km in near physiological conditions. |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ | |