Induction of osteoconductivity by BMP-2 gene modification of mesenchymal stem cells combined with plasma-sprayed hydroxyapatite coating |
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Authors: | Jiang Wu Ying-qiang Guo Guang-fu Yin Huai-qing Chen |
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Affiliation: | a College of Material Science and Engineering, Sichuan University, Chengdu 610064, China b Institute of Biomedical Engineering, West China Medical Center of Sichuan University, Chengdu 610041, China c Department of Cardiovascular Surgery, West China Hospital of Sichuan University, Chengdu 610041, China |
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Abstract: | Success in bone implant depends greatly on the composition and surface features of the implant. The surface-modification measures not only favor the implant's osteoconductivity, but also promote both bone anchoring and biomechanical stability. This paper reports an approach to combine a hydroxyapatite (HA) coated substrate with a cellular vehicle for the delivery of bone morphogenetic protein-2 (BMP-2) synergistically enhancing the osteoconductivity of implant surfaces. We examined the attachment, growth and osteoinductive activity of transfected BMP-producing bone marrow mesenchymal stem cells (BMSCs) on a plasma-sprayed HA coated substrate. It was found that the HA coated substrate could allow the attachment and growth of BMP-2 gene modified BMSCs, and this combined application synergistically enhanced osteconductivity of the substrate surface. This synergistic method may be of osseointegration value in orthopedic and dental implant surgery. |
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Keywords: | 87.68.+z |
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