Protein fragment imaging using ink jet printing digestion technique |
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Authors: | Manabu Komatsu Yohei Murayama Hiroyuki Hashimoto |
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Institution: | Canon Research Center, Canon Inc., 3-30-2 Shimomaruko, Ohta-ku, Tokyo 146-8501, Japan |
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Abstract: | We have developed a capable tool for visualizing protein distribution at the sub-cell level with TOF-SIMS. This new method of visualizing a protein includes: (1) printing a digestive enzyme with ink jet (IJ) printing technology onto a tissue section, (2) measuring the two-dimensional distributions of decomposed peptide ions derived from digested protein on the tissue section by TOF-SIMS with a bismuth cluster ion (Bi3+) source and using the aqueous ion enhancement method, and (3) analyzing the two-dimensional distributions of proteins using the results of proteome analysis. We have not yet tried this method onto a real tissue section. To show the possibility of this method, TOF-SIMS results of visualizing dot-patterned digested fragment peptide ions using IJ printing digestion technique onto a protein film are presented. |
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Keywords: | Protein imaging Digestive enzyme Aqueous-ion-enhancement Bubble jet printing |
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