Principles and theory of two-cycle circulation liquid chromatography

The principle of cycle repetition with reversal of two columns is extended to two-cycle circulation chromatography as a version of preparative liquid chromatography. This is regarded as an alternative to closed-loop circulation. A theory of fine separation of binary mixtures is developed taking into account the possibility of programming the length of the sorbent layer and stepwise chromatography. Theoretical analysis and calculations have shown that circulation systems with two cycles and columns filled with sorbent particles ranging from 10 to 35 m are characterized by high resolution and a relatively low pressure drop between the columns.Translated fromIzvestiya Akademii Nauk. Seriya Khimicheskaya, No. 9, pp. 1535–538, September, 1993.