| α-FACTOR-DIRECTED SYNTHESIS AND SECRETION OF β-SUBUNIT OF HUMAN CHORIOGONADOTROPIN (β-hCG)IN YEAST S. cerevisiae |
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| 作者姓名： | 唐鹰 匡达人 吴瑞 |
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| 作者单位： | Shanghai Institute of Cell Biology，Academia Sinica Cornell University. Ithaca，USA，Shanghai Institute of Cell Biology，Academia Sinica Cornell University. Ithaca，USA，Shanghai Institute of Cell Biology，Academia Sinica Cornell University. Ithaca，USA |
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| 基金项目： | Proiect supported by the National Natural Science Foundation of China. |
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| 摘 要： | A couple of sbuttle vectors (pYNA and pYNB), containing a sequence coding for the leader regionof the α-factor precursor (α-factor leader sequence) and the origin of 2μm circle, were constructed. ThecDNA for the β-hCG (145aa) was fused in phase to the 3'-end of the α-factor leader sequence andtransformed into the yeast Saccharomyces cercvisiae. The yeast cells carrying this hybrid gene synthesizedand secreted a protein with the immunoactivity of β-hCG in an amount of 45μg per liter of culture. Thepreliminary characterization of this protein is also reported in this paper.
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