Amperometric hydrogen peroxide sensor based on a sol-gel-derived ceramic carbon composite electrode with toluidine blue covalently immobilized using 3-aminopropyltrimethoxysilane |
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Authors: | K Thenmozhi S Sriman Narayanan |
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Institution: | (1) Department of Analytical Chemistry, University of Madras, Guindy Campus, Chennai, 600 025, India |
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Abstract: | A carbon composite amperometric hydrogen peroxide sensor has been developed using a sol-gel technique. Toluidine blue (TB),
which acts as the redox mediator, was covalently immobilized via glutaraldehyde crosslinking with an organically modified
silane, namely 3-aminopropyltrimethoxysilane (APTMOS). Methyltrimethoxysilane (MTMOS) was used as the additional monomer;
this controls the hydrophobicity of the electrode surface, thus limiting the wettability. The immobilization of TB within
the sol-gel matrix was confirmed with FTIR studies. The sol-gel mixture containing TB immobilized in APTMOS and MTMOS was
mixed with graphite powder in order to prepare the carbon composite electrode. The electrode was characterized using voltammetric
techniques and its electrocatalytic activity for the reduction of hydrogen peroxide was also studied. The carbon composite
electrode has the advantage of sensing H2O2 at a lower potential and with a higher sensitivity, and interferences due to ascorbic acid, uric acid and acetaminophen were
greatly minimized. The linear range for the determination of H2O2 extends from 5.37 × 10−6 to 6.15 × 10−3 M, with a correlation coefficient of 0.9981. The detection limit was found to be 2.15 × 10−6 M. The covalent immobilization of TB effectively prevents the leakage of the water-soluble mediator during measurements.
The modified electrode, aside from electrocatalyzing the reduction of H2O2, exhibits distinct advantages in terms of surface renewal in the event of surface fouling, as well as simple preparation,
good chemical and mechanical stability, and good reproducibility.
Figure Amperometric hydrogen peroxide sensor based on sol-gel-derived ceramic carbon composite electrode with toluidine blue covalently
immobilized using 3-aminopropyltrimethoxysilane
Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users. |
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Keywords: | Sol-gel Covalent immobilization Toluidine blue Amperometry Hydrogen peroxide |
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